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Fig. Supp. 5 A) left: Brightfield images of calb2a + calb2b sgRNA injected larvae at 3 days post fertilization (dpf), showing a severe phenotype (top) and a mild/mosaic phenotype (bottom). Right: Confocal images of dmrt3aGFP in severe (top) and mild/mosaic (bottom) phenotypical larvae. B) Genomic FLA primers. C) Table displaying the PCR fragment lengths detected following FLA-analysis for two control larvae (controls) and four phenotypical sgRNA injected larvae (crispants). Fragment Lengths found in control larvae are made bold.
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Reprinted from Developmental Biology, 508, Iglesias Gonzalez, A.B., Koning, H.K., Tuz-Sasik, M.U., van Osselen, I., Manuel, R., Boije, H., Perturbed development of calb2b expressing dI6 interneurons and motor neurons underlies locomotor defects observed in calretinin knock-down zebrafish larvae, 778777-87, Copyright (2024) with permission from Elsevier. Full text @ Dev. Biol.