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Fig. 2 Disruption of the nr2f genes by the CRISPR/Cas9 technique. The functional domains or the N-terminal region of nr2f genes was disrupted by co-injection of gRNA and Cas9 protein. (a–c) The target sequences (shown in red) with adjacent PAM sites (yellow) are shown with the flanking sequences for the HMA primers (HMA-s and HMA-as). (a′–c′) Schematic views showing the structures of wild-type and mutant Nr2f proteins. The DNA-binding domains, ligand-binding domains, Nr2f sequences, and nonsense sequences due to frameshift are shown in red, blue, gray, and white, respectively. The target sites are shown with open triangles. The sizes of the gene products are shown on the right with those of wild-type Nr2f sequences in parentheses.