ZFIN Image: Diana et al., 2024, Figure 6

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Figure Caption

Figure 6

Smpx is required for normal kinocilium structure. Immunofluorescence performed on whole mount 120 hpf larvae with an antibody against acetylated tubulin to label the neuromast kinocilia. (A,B) Compared to controls (ctrlMO), the kinocilia of morphant larvae (smpxMO) appear clearly shorter in length and numerically less dense. (C,D) As for the knockdown experiment, when compared to controls (ctrl), the kinocilia of crispant larvae (smpx^crispant) are shorter and numerically lower. (A,B) ctrlMO n = 5, smpxMO n = 4. (C,D) ctrlMO n = 5, smpxMO n = 5. (A–D) Confocal Z-stack images of neuromast L4 for each larva. n is equal to the sum of the larvae from two independent experiments. Embryos were counterstained with DAPI to visualize cell nuclei. Scale bars are 6 µm.

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Phenotype details

Acknowledgments

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