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Fig. 4 Effect of GCSW210 on gene expressions in HG-mediated insulin resistance model of zebrafish larvae. A) insa; B) irs1; C) akt; D) ampka. Zebrafish larvae were exposed to d-glucose solution (130 mM) for 72 h starting 5 dpf to induce IR, followed by treatment with GCSW210 (250 μg/mL) for 24 h and homogenization. Data are presented as mean ± SE (n = 3) and analyzed by one-way analysis of variance. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus control; #p < 0.05, ##p < 0.01, ###p < 0.001 versus HG. Abbreviations: GC; subcritical-water extract of Gracilaria chorda at 210 °C (GCSW210), HG; High glucose.