|
Fig. 1 Effect of GCSW210 on toxicity in and glucose uptake of zebrafish larvae. A) Indications of toxicity; B) survivability; C) fluorescence imaging of glucose uptake; D) level of glucose uptake. Fertilized eggs were collected and placed in 96-well culture plates. After 7 days post-fertilization (dpf), larvae were treated with GCSW210 (125 and 250 μg/mL) for 24 h post-fertilization (hpf), and mortality and malformation were observed. All values are presented as mean ± SE from three independent repeated experiments and analyzed by one-way analysis of variance followed by Dunnett test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. control. Abbreviations: GCSW210; subcritical-water extract of Gracilaria chorda (GC) at 210 °C, dpf; days post-fertilization, hpf; hours post-fertilization.