ZFIN Image: Nwagbo et al., 2024, Fig. 7

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Fig. 7

Gross histology and ORO stain. Toluidine blue-stained retinal sections of 1-year-old wild-type and homozygous Elovl4b KO fish. We observed no significant differences in the gross morphology and lamination of the retinas from both groups. Images were taken at 4× (A) and 40× (B) magnification. Cryosections of 1-year-old age-matched male wild-type and homozygous Elovl4b KO zebrafish retinas, treated with ORO to stain lipids (red) and counterstained with hematoxylin (blue) (C). The red arrows point to lipid-rich ORO-stained puncta localizing in the basal RPE of homozygous Elovl4b KO mutants. Images were taken at 40× with a Zeiss AxioScan slide scanner. GCL, retinal ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; OS, outer segment; RPE, retinal pigment epithelium.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.