ZFIN Image: Nwagbo et al., 2024, Fig. 2

Image

Figure Caption

Fig. 2

Sequencing and PCR analysis. A: Gel electrophoresis showing wild-type, heterozygous, and homozygous Elovl4b KO zebrafish. DNA was extracted from caudal fin clips and amplified using PCR. The PCR product was subsequently run on 2% agarose gel at 110 V. This image was captured using an iBright CL750 imaging system (Invitrogen, Carlsbad, CA). B: Nucleotide sequence alignment between wild-type and homozygous Elovl4b mutant fish shows that a 56 bp deletion mutation at exon 2 of zebrafish Elolv4b leads to a premature termination codon at position 5 of the protein-coding sequence. The start codons are highlighted in yellow, and the premature termination codon is highlighted in green.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.