Figure 9

Figure 9

fbln1^−/− mutant zebrafish show missing opercle on the right side. (A) Adult fbln1^−/− fish bright field image showing the missing opercle (yellow arrow). (B) 3D Reconstructed images from µCT scans of adult WT control and fbln1^−/− fish head (yellow arrow: missing opercle, blue arrow: thickened subopercular bone). (C) 10 dpf Alizarin red stained zebrafish in ventral view (yellow arrow: missing opercle). (D) Left (L) and Right (R) opercle area measured in ventral view on 10 dpf alizarin red stained WT control and fbln1^−/− zebrafish larvae show the trend of asymmetry between the L and R opercles in fbln1^−/− mutant zebrafish (n = 34 WT, n = 37 fbln1^−/− mutants). (E) Differentially expressed genes in fbln1^−/− mutants that are involved in Fgf and Mapk signaling.