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Fig. 7 Fgf signal inhibition can partially correct the malformation of the posterior cartilages in nkx2.3 morphants. (A) Embryo morphology following SU5402 treatment on nkx2.3 morphants. We tracked the embryos' growth and took pictures of them at 4 dpf. Embryos treated with 2.5 μM SU5402 between 24 and 48 hpf after receiving an injection of nkx2.3 cMO or nkx2.3 MO1 are shown in the representative images. Scale bars: 200 μm. (B) Alcian blue staining of nkx2.3 morphants treated with SU5402 and the statistic results. At 4 dpf, the embryos of nkx2.3 morphants and controls that had been treated with of 2.5 μM SU5402 were collected and stained with Alcian blue. Representative images of the various pharyngeal cartilage classes were shown in the left panel. Scale bars: 100 μm. The bar graph on the right displays the proportion of each type of embryo seen in the left panel in each treatment group.