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Fig. 8 4C4 expression was evaluated in the PPN 30 days after spontaneous nicotine withdrawal. Representative images obtained from coronal sections show 4C4 immunofluorescence in (a) control (drug-free water + water), (b) nicotine + water, (c) water + fluoxetine and (d) nicotine + fluoxetine. Insets show representative examples of microglial cells. (e, f) Quantitative analysis. No change in 4C4 immunofluorescence area (e) nor in morphological parameters (f) are shown (here major is shown: the major axis of the best fitting ellipse of the analysed cell) (e) nor in 4C4 fluorescence intensity (f). Data are mean ± SEM of the mean of 3/7 zebrafish/group (25–88 cell/group) (one-way analysis of variance (ANOVA)). Each dot represents one animal. (one-way ANOVA).