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Fig. 5 Loss of Islet + cranial motor neurons in plxna4 mutants. WT and plxna4ca307 hindbrain sections immunolabeled for Islet1 at 24 hpf (A, B) and 48 hpf (D, E). Ventrally located motor neurons can be seen in both genotypes (arrows, dotted yellow lines). (C) Blinded quantitation of Islet1 positive cells in the transverse sections of the hindbrain of 24 hpf WT and plxna4 mutant embryos. WT N = 4, n = 9; −/− N = 4, n = 11; P = 0.3640, unpaired Student's t-test. (F) Blinded quantitation of Islet1 positive cells in the hindbrain of 48 hpf plxna4 WT and mutant embryos in the hindbrain (N = 3; wt n = 7; −/− n = 8; P = 0.0480, unpaired Student's t-test), anterior spinal cord when dorsally located Rohon–Beard sensory neurons appear (N = 3, WT n = 7; −/− n = 7; P = 0.1197 Unpaired Student's t-test), and the spinal cord (N = 3; WT n = 7; −/− n = 5; P = 0.4526, unpaired Student's t-test). D: dorsal, ov: otic vesicle, V: ventral. Scale bar in A is 50 μm.