Fig. 6

Fig. 6

NO signaling regulates CM migration during ventricle regeneration. A–C Whole-mount in situ hybridizations showed reduced expression of snail1a, twist1a and vimentin at 72 hpt in control and ablated hearts treated with DMSO, HC-067047 or L-NMMA for 48–72 hpt. Numbers indicate the ratio of representative staining observed. D–F Injection of myl7:lifeact-eGFP plasmid into Tg(vmhc:mCherry-NTR) embryos at the one-cell stage to monitor the migratory behavior of single eGFP⁺ CMs in the ventricles or atriums of ablated hearts treated with DMSO, HC-067047 or L-NMMA for 48–72 hpt. G Quantification of the cell migration rate at 96 hpt in ablated hearts treated with DMSO, HC-067047 or L-NMMA for 48–72 hpt. The numbers of larvae analyzed for each condition are indicated. Binomial test, ****P < 0.0001. H Time series images showed the migration process of an eGFP⁺ CM in the ablated heart at 48–96 hpt. Scale bars, 50 μm. Dashed lines outline the hearts. hpt hours post treatment, CM cardiomyocyte, V ventricle, A atrium, HC HC-067047