Fig. 1 Anatomy and histology of OFTs in actinopterygians. (a) Phylogeny of actinopterygian species. (b) Anatomy and histology of OFTs in representative actinopterygian species. Left panels are results of Elastica van Gieson staining, which visualizes the accumulation of elastic fibres. Note that the elastic fibres of BAs in teleosts are abundant, while those of CAs in non-teleost fishes are restricted to the inner lining. Right panels are results of double immunohistochemistry against α-sarcomeric actinin (cardiac muscle, green) and myosin light-chain kinase (smooth muscle, magenta). Note that teleost BAs are composed of smooth muscle, while CAs in non-teleost fish are composed of cardiac muscle. The phylogenetic timing of acquisition of BA is coincident with 3R WGD. CA, conus arteriosus; V, ventricle. Scale bars, 400 μm.
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