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Fig. 4 Trim33 deficiency affects the survival of primitive macrophages and production of definitive macrophages cell-autonomously. (A-C) Lateral view of the caudal regions of parabiotes fused by the head. (A) Wild-type Tg(mpeg1:eGFP-F) control embryo (right) fused to a monTB222/Tg(gata1:Dsred; mfap4:turquoise) sibling embryo (left) at 48 hpf. (B) Wild-type Tg(mpeg1:eGFP-F) control embryo (right) fused to a monTB222/Tg(gata1:Dsred; mfap4:turquoise) mutant embryo (left). Top images show Gata1:DsRed fluorescence in white, on a bright-field background; bottom images show mpeg1:GFP in green, and mfap4:turquoise in magenta. (C) Mutant mfap4:turquoise+ macrophages in the wild-type tail of the parabiosis (left panels) and wild-type mpeg1:eGFP-F+ macrophages in the monTB222 mutant tail of the parabiosis (right panels) at 2, 4 and 5 dpf. Sib, sibling; Mut, mutant.