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Fig. 1 plxnd1 mutants have misaligned ISLVs and aberrant facial lymphatic branches. (A-D′) Confocal images of the trunk (A-A″,B-B″) and facial (C,D) lymphatics in lyve1b:DsRed (white); kdrl:EGFP (green) larvae at 6 dpf. Yellow arrowheads indicate ISLVs that are not aligned with an ISV (B,B″) or aberrant facial lymphatic branches (D) in plxnd1nz75 larvae. For clarity, EGFP-positive vessels in the head are not displayed. Schematics highlight the misaligned ISLVs (A‴,B‴) or aberrant facial lymphatics (C′,D′) in each corresponding confocal image. (E,F) Quantitation of the number of misaligned ISLVs (E) and aberrant facial lymphatic branches (F). P>0.05 (n.s., not significant), *P<0.05, **P<0.01 (Brown-Forsythe, and Welch ANOVA and Kruskal–Wallis test); data are mean±s.d. Scale bars: 50 µm. ISLV, intersegmental lymphatic vessel. Numbers in graphs represent numbers of larvae.