|
Fig. EV5 Dose–response effect of the five favorite Hits on the restoration of motility A–E. Treatment with Aceclidine Hydrochloride (Acecl.) (A), Digitoxigenin (Digito.) (B), Oxymetazoline Hydrochloride (Oxymet.) (C), Trichlormethiazide (Trichlor.) (D) and Phentolamine Hydrochloride (Phento.) (E) was performed from 0.1 to 50 μM. Data information: Data are not provided for the higher doses (30 and 50 μM) due to toxicity. Results show the distance traveled at 5 dpf, during 1 h by gan MO-injected larvae, treated with either DMSO or Hits from 0.1 to 10 μM. Each dot represents individual values, (A) n = 25 (MO + DMSO), n = 5 (MO + Acecl. 0.1 μM), n = 5 (MO + Acecl. 1 μM), n = 5 (MO + Acecl. 2 μM), n = 7 (MO + Acecl. 5 μM), n = 7 (MO + Acecl. 10 μM); (B) n = 25 (MO + DMSO), n = 5 (MO + Digito. 0.1 μM), n = 5 (MO + Digito. 1 μM), n = 6 (MO + Digito. 2 μM), n = 7 (MO + Digito. 5 μM), n = 5 (MO + Digito. 10 μM); (C) n = 25 (MO + DMSO), n = 5 (MO + Oxymet. 0.1 μM), n = 2 (MO + Oxymet. 1 μM), n = 5 (MO + Oxymet. 2 μM), n = 10 (MO + Oxymet. 5 μM), n = 6 (MO + Oxymet. 10 μM); (D) n = 25 (MO + DMSO), n = 5 (MO + Trichlor. 0.1 μM), n = 6 (MO + Trichlor. 1 μM), n = 4 (MO + Trichlor. 2 μM), n = 6 (MO + Trichlor. 5 μM), n = 6 (MO + Trichlor. 10 μM); (E) n = 25 (MO + DMSO), n = 4 (MO + Phento. 0.1 μM), n = 3 (MO + Phento. 1 μM), n = 5 (MO + Phento. 2 μM), n = 4 (MO + Phento. 5 μM), n = 7 (MO + Phento. 10 μM). In the absence of normality of distribution of the data, a nonparametric Kruskal–Wallis test is applied; means ± SEM are represented. *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001.