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Fig. 2 Zygotic smyhc1 mutation reduces embryo motility but survive to adulthood. A) Representative bright-field images of 1,2,3,4 and 5 dpf larvae from smyhc1kg179/+ and smyhc1kg180/+ heterozygote in-crosses. Fish are shown anterior towards the left and dorsal upwards with genotyped heterozygotes and mutants below their respective wild type siblings. Scale bars = 0.5 mm. B) Randomly selected larvae were dechorionated at 24 hpf and examined for presence or absence of tail coiling movement in smyhc1kg179/+ in-cross (n = 82) and in smyhc1kg180/+ in-cross (n = 52). The genotype of the fish was determined after the examination by DNA sequencing. Fish derived from several in-crosses of smyhc1kg179/+ (n = 101) and smyhc1kg180/+ (n = 100) fish were reared with their siblings and genotyped at 4 months post-fertilization (mpf). C) Adults showed the expected Mendelian ratios at 4 mpf. Fish numbers above each bar. D) Adults at 12 mpf. Scale bars = 1 cm. E) Length and mass at 4 mpf of genotyped siblings showed no significant difference between genotypes. Small symbols indicate individuals, large symbols the mean for each sex and genotype ± S.E.M. Overall length and weight were less in adult fish from the smyhc1kg180/+ lay compared to adult fish from the smyhc1kg179/+ lay, a difference that may reflect an uncontrolled environmental or genetic background effect.
Reprinted from Developmental Biology, 499, Hau, H.A., Kelu, J.J., Ochala, J., Hughes, S.M., Slow myosin heavy chain 1 is required for slow myofibril and muscle fibre growth but not for myofibril initiation, 47-58, Copyright (2023) with permission from Elsevier. Full text @ Dev. Biol.