Figure 4

Figure 4 Loss of Flii results in a reduced number of cardiac trabeculae.

(A) Single confocal planes of representative Tg(myl7:LIFEACT-GFP) ventricular outer curvatures at 96 hpf through 6 dpf of flii^+/? siblings (left) and flii^{D110fs/D110fs} mutants (right). The 96 hpf hearts express Tg(myl7:nDsRed2) to visualize nuclei. Yellow false color depicts outer curvature. Trabecular myocardium is highlighted in blue. Scale bars: 25 μm. (B) Cropped areas corresponding to white rectangles in A. The dotted lines depict the compact layer of cardiomyocytes. (C) Quantification of protruding cardiomyocytes out of the single-layered compact myocardium in the ventricular outer curvature from sagittal planes at 96 hpf through 6 dpf. Unpaired t test; values represent means ± SEM; (96 hpf flii^+/? siblings n = 6, flii^{D110fs/D110fs}n = 9); (120 hpf flii^+/? siblings n = 5, flii^{D110fs/D110fs}n = 5); (6 dpf flii^+/? siblings n = 3, flii^{D110fs/D110fs}n = 3).