Fig. 2

Fig. 2

Histaminergic, dopaminergic, and hcrt systems in hrh1^−/− zebrafish. a Representative pictures (ventral view) for immunohistochemistry of histamine and Th1 in hrh1^+/+ and hrh1^−/− 6 dpf zebrafish brains. Anterior to the left. Scale bars: 100 µm. b Histaminergic neuron numbers. N = 6. c Number of Th1-expressing neurons in group 10. N = 6. d–f qPCR results of th1, th2, chata in hrh1^+/+ and hrh1^−/− in 6 dpf whole zebrafish. N (hrh1^+/+) = 5, N (hrh1^−/−) = 6. g–h Representative pictures (ventral view) for in situ hybridization of hcrt in hrh1^+/+ and hrh1^−/− 6 dpf zebrafish brain and cell numbers. Anterior to the top. Scale bar 50 µm. N = 6. i qPCR results of hcrt in hrh1^+/+ and hrh1^−/− in 6 dpf whole zebrafish. N (hrh1^+/+) = 5, N (hrh1^−/.⁻) = 6. All histograms are mean ± SEM, unpaired t-test, two-tailed. *0.01 < P ≤ 0.05; **0.001 < P ≤ 0.01; ***0.0001 < P ≤ 0.001; ****P ≤ 0.0001