Fig. 1

Phenotypes of keap1a;keap1b-DKO zebrafish. (A) Survival rates of offspring from heterozygous keap1a;keap1b-DKO (Ka1Kb1) parents at 7, 14, and 21 dpf. The red bar represents homozygous keap1a;keap1b-DKO (Ka0Kb0) larvae. (B) Survival rates of 3-mpf offspring from Ka1Kb1 parents (left) and Ka0Kb1 parents (right). WT, −/+, and −/− indicate wild-type, heterozygous, and homozygous fish, respectively. Data from 3 independent experiments were combined. (C) Eating analysis of 5- to 10-dpf offspring from Ka0Kb1 parents. The numbers at the top are the number of larvae tested. It was noted that eating in Ka0Kb0 larvae was reduced. The black arrowheads in the right panels indicate the food inside the gut of the larvae. (D) Hematoxylin and eosin staining of paraffin sections of 7-dpf larvae. In the right panel, the larval digestive tract is indicated with red dotted lines, and vertical sections at the locations of the esophagus (e, green line), the esophagus-gut junction (j), and the intestinal bulb (i) are shown on the left panel. Scale bars, 50 μm. (E) Alcian blue staining of head cartilages of 7-dpf larvae. (F) Survival rates of offspring from Ka0Kb1 parents without feeding. Survival was observed every 24 h from 6 to 11 dpf. The tested numbers were as follows: Ka0Kb2 n = 18, Ka0Kb1 n = 40, Ka0Kb0 n = 18. The asterisks indicate significant differences (**p < 0.01). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)