ZFIN Image: DeMoya et al., 2023, FIGURE 6

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Figure Caption

FIGURE 6

Lineage tracing reveal changes to SHF in MZsap130a at 48hpf (A) Diagram showing how the FHF heart tube at 24hpf was photoconverted to red, leaving SHF progenitors unlabeled in green and imaging at 48hpf. (B, C) Confocal imagines of Tg(nkx2.5:kedge) and MZsap130a;Tg(nkx2.5:kedge) at 48hpf with the heart tube being photoconverted at 24hpf, the red outlined region represents area measurements collected. (D) Quantified SHF (green area) accreted by 48hpf, pval is from a one ANOVA, error bars are SEM. Each point represents a single embryo, Tg(nkx2.5:kedge), n = 15; MZsap130a;Tg(nkx2.5:kedge) NV, n = 14; MZsap130a;Tg(nkx2.5:kedge) SV, n = 10. V and A are ventricle and atria, respectively. Scale bar 100 μm.

Acknowledgments

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