ZFIN Image: Shi et al., 2023, Figure 2

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Figure Caption

Figure 2

Identification of chd7 knockout zebrafish. (a) Schematic of the Cas9-sgRNA targeted site located in the first exon of chd7. (b) After crossbreeding and self-crossing, homozygous mutant strains were obtained. F0: sgRNA/Cas9 microinjected zebrafish was mated with WT zebrafish; F1: the heterozygous descendant of F0, which have the same type of mutation; F2: homozygous descendant of F1. (c,d) Sequencing results showed that 16 bp were deleted in the chd7^−/− zebrafish. (e) Schematic of the protein sequence showed that CHD7 mutated zebrafish expressed truncated CHD7 composed of 200 amino acids, while wild-type CHD7 is composed of 3140 amino acids.

Acknowledgments

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