Fig. 5

(A) Aspect ratio was measured at 11 hours post fertilization (hpf) as the ratio of a divided by b, shown on the embryo schematic. WT and hypomorphic variants reduce the aspect ratio, loss-of-function (LOF) variants have no effect, and dominant-negative (DN) variants elongate the embryo. (B) Hierarchical clustering of the quantitative phenotypic metrics ranks DN alleles as closer to MAP4K4^WT in phenotypic output than to loss of function alleles. The P value for each measurement is shown in the box, with the absence of symbols indicating nonsignificant changes. Graph: pale gray, vehicle; dark gray, MAP4K4^WT; purple, missense variants; green, truncation variants. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by pairwise Student’s t test compared to vehicle. ANOVA gives P < 0.0001 for (A). n numbers are inset to graph in gray text. Error bars represent the SEM.