ZFIN Image: Weinschutz Mendes et al., 2023, Fig. 1

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Figure Caption

Fig. 1 ASD gene expression in zebrafish and mutant generation

(A) Zebrafish mutant lines. See Table S and Figures S1A–S1I for mutant sequences.

(B) Whole-mount RNA in situ hybridization of ASD gene orthologs in zebrafish at 24 and 48 hpf. For cntnap2a and cntnap2b, see Hoffman et al. (2016).²¹ Lateral views are shown in the left three panels; dorsal views are shown in the right panel. Anterior to the left; tel, telencephalon; di, diencephalon; MHB, midbrain-hindbrain boundary; OT, optic tectum; HB, hindbrain. Scale bars, 0.1 mm.

(C) Mapping zebrafish RNA-seq datasets³⁰ to the human BrainSpan developmental transcriptome RNA-seq dataset ³¹ using principal component analysis.¹³ Principal component 1 (PC1) is plotted. Bands represent the 95% confidence interval of the mean PC1 value for human developmental stages. Biological replicates of zebrafish samples are shown as points. pcw, postconception weeks; hpf, hours post-fertilization; dpf, days post-fertilization. See Figure S1L for the full PCA

Acknowledgments

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