Fig. 1

The effect of crb2a^−/− on retinal neurogenesis and lamination. Characterisation of cell types present in zebrafish retina at 56 hpf (A–H, wild type zebrafish, A′-H′ crb2a^m289 zebrafish). Early retinal progenitor cells were identified through staining with pax6 in WT (A and B) and crb2a^−/− (A′ and B′) and anti-vsx2 (C, D, C′ and D′) at 56 hpf. Anti-ZPR1 antibody (zpr1) was used to identify the presence of cone cells (E and F) in WT; however these were absent from the mutant retina (E′ and F′) at 80 hpf. The presence of Müller cells was visualised using an anti-glutamate synthetase antibody (GS) in WT (G and H) and mutant (G′ and H′) retina. M-phase nuclei, visualised using an anti-phospho-Histone 3 antibody (PH3), were observed in both WT (I) and crb2a^−/− (J) at 56-hpf. All nuclei are stained with DAPI. S-phase nuclei as visualised by 5-ethynyl-2’-deoxyuridine (EdU) incorporation were observed in WT (I′) and crb2a^−/− (J′) retina. Panels I″ and J″ are merged images of previous panels. (K) Quantification of PH3-positive cells; *** p < 0.001. Scale bar, 50 μm.