ZFIN Image: Lin et al., 2023, Fig. 7

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Figure Caption

Fig. 7

YULINK-knockdown dampens PI3K signaling pathway downstream of VEGFR2. A Western blots of total cell lysates isolated from YULINK-knockdown and vector control HUVECs. Phosphorylation (indicating activation) of VEGFR2 and PI3K subunit P85 was analyzed, with VEGFR2 and PI3K subunit P85 serving as the respective internal controls. GAPDH was detected as a loading control. Band intensities were quantified using the ImageQuant TL software (GE Healthcare), normalized relative to the quantity of their respective control, and expressed as percentages of the value. B Phosflow analysis of phosphor-AKT (Ser473) levels in YULINK-knockdown (red) and vector control (blue) HUVECs. An average of 3,000 gated events was collected on a Canto flow cytometer. Data were analyzed using DIVA software. The dashed profile indicates background staining with a control IgG mAb. Total AKT served as the internal control

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