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FIGURE 5
Differentiation ability of SHSY-5Y cells and IPSCs with different genotype. (A) Differentiation of SHSY-5Y cells were induced by ATRA and ENCCs were obtained from IPSCs by adding LDN193189 and SB431542 to the differentiation medium. (B) Immunofluorescence staining of a SHSY-5Y differentiation marker NSE and bright field morphology of SHSY-5Y cells with different genotypes. (C) Semi-quantitative analysis of a SHSY-5Y differentiation marker NSE in SHSY-5Y cells with different genotypes. (D) Flow cytometry analysis of the ENCC differentiation rate (HNK-1+ p75NTR+) of IPSCs with different genotypes. (E) Luciferase assay showed that CTCF had significantly higher binding ability to SNP rs2439302 CC than GG in SHSY-5Y cells.