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Figure 6- supplement 1 Klhl40a deficiency does not affect protein levels of autophagy markers in skeletal muscle. (A) Examination of autophagy markers in the proteome of controls and klhl41a mutant skeletal muscle at 1.5 months and 3 months of age (ns: nonsignificant). (B) Western blot of control and klhl40a skeletal muscle extracts with LC3B antibody. (C) Quantification of the LC-II/LC-I ratio by Western blot analysis. (D) RT-PCR assay for xbp1 transcripts in control and klhl41a mutant skeletal muscle. (E) Western Blot and protein quantification of Perk and P-Perk in control and klhl41a mutant skeletal muscle. Data are mean ± S.E.M (unpaired t-test, parametric).