Fig. 1

Fig. 1 Fig. 1. TCC induces hyperaemia in the joint of the zebrafish anal fin. (A) Typical images reveal that TCC induces hyperaemia in the reciprocal of the zebrafish anal fin in a time- and dose-dependent manner. The zebrafish were soaked in 0.03–3 μM TCC for different periods, and the same dose of DMSO (0.025 %, V/V) was employed as a control. The black arrows point to the hyperaemia area, and the number in the frame refers to the number of zebrafish as shown in the image/total number of zebrafish involved in this group. Experiments were performed three times with similar results. (B) Quantification of the hyperaemia in the joint of the zebrafish anal fin according to the virtual ruler in the software of the imaging system. L0 represents the length of the whole anal fin, while Lh represents the length of the hyperaemia area. The hyperaemia rate is presented as 100 % X (Lh/L0); (C) Quantification of the time- and dose-dependent hyperaemia in the joints of zebrafish anal fins induced by TCC. * indicates P < 0.05, * * indicates P < 0.01, * ** indicates P < 0.001, * ** * indicates P < 0.0001. The comparison was performed between the TCC-treated group and the DMSO (0.025 %, V/V) control at the same time point.