ZFIN Image: Fetsko et al., 2022, Fig. 5

Image

Figure Caption

Fig. 5

Fig. 5. Vegf signaling is not required for Wnt/?-catenin transcriptional activity. (A) Representative confocal images of tcf:EGFP, kdrl:mCherry embryos at 2 dpf that were untreated, DMH4-treated (DMH4), or injected with a gpr124 morpholino (gpr124 MO). tcf:EGFP signal is present in the WT brain vasculature (top panels) and in the PHBCs of the DMH4-treated embryos (middle panels; white arrows), but not the gpr124 MO-injected embryos (bottom panels). Scale bar is 100 ??m for the first three columns and 40 ??m for the zoomed images (right panels). (B) Quantification of the fraction of kdrl:mCherry-labeled hindbrain vasculature expressing tcf:EGFP. (C) Quantification of the normalized relative fluorescence intensity (RFI) of tcf:EGFP in the hindbrain vasculature. Data in B and C are presented as means (n ?= ?4) ?� ?SEM (?p ?< ?0.05; ???p ?< ?0.001; ns ?= ?not significant).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 494, Fetsko, A.R., Sebo, D.J., Taylor, M.R., Brain endothelial cells acquire blood-brain barrier properties in the absence of Vegf-dependent CNS angiogenesis, 46-59, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.