FIGURE 4

FIGURE 4

Increased in vitro angiogenesis upon TDP-43 KD in HUVEC and identification of mis-regulated pathways by NGS. (A) KD of TDP-43 in HUVEC with two different shRNAs, shTARDBP#1 and #2 reduces TDP-43 protein levels. shCtr transduced HUVEC serve as control, each lane represents a biological replicate, Calnexin serves as loading control. (B) Images show tubular network formation of representative fields of view of shCtr, shTARDBP#1, and shTARDBP#2 transduced HUVEC (magnification: ×10). Bar graph displays the quantified connections per field of view in TDP-43 KD with shTARDBP#1 and shTARDBP#2 compared to shCtr. D’Agostino-Pearson omnibus normality test and one-way ANOVA, n ≥ 8 in three independent experiments. (C) Selection of NGS hits of HUVEC upon TDP-43 KD with fold change and adjusted p-value. (D) Immunoblot validation of FN1, ITGA4, and ITGB1 upon KD of TDP-43 with shTARDBP#1 and shTARDBP#2 in comparison to shCtr transduced cells. Each lane represents one biological replicate. The band intensities of the indicated bands (arrowhead) have been quantified using ImageJ, t-test n = 3.