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Figure 6—figure supplement 1. Co-localization of the pituicyte marker cyp26b1 and vegfaa or vegfab BAC transgenic reporter expression at early larval stages.

(A–A”) Single confocal z-plane images of 75 hours post fertilization (hpf) TgBAC(vegfaa:EGFP) ventral brain following in situ hybridization of cyp26b1, showing overlapping signals between the EGFP+ cells and cyp26b1+ pituicyte (n=10). Yellow arrowheads indicate the overlapping cells. (B–B”) Single confocal z-plane images of 75 hpf TgBAC(vegfab:EGFP) ventral brain following in situ hybridization of cyp26b1, showing overlapping signals between the EGFP+ cells and cyp26b1+ pituicyte (n=11). Yellow arrowheads indicate the overlapping cells.

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