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Figure 5
Hemoglobin, oxygen consumption and locomotor activity. (A) O-dianisidine staining for hemoglobin in foxO4−/− and WT zebrafish embryos from 36 hpf to 96 hpf. Scale bar = 250 µm. (B) The ratio of total strength of foxO4−/− zebrafish staining signal to WT. ImageJ software was used to quantify the total strength of the staining signal of each embryo. (C) Oxygen consumption of foxO4−/− and WT adult zebrafish. (D) Locomotor activity of foxO4−/− and WT zebrafish embryos and adults. * p < 0.05, ** p < 0.01, *** p < 0.001.
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