ZFIN Image: Keatinge et al., 2023, Fig. 2.

Image

Figure Caption

Fig. 2.

Genetic and biochemical characterisation of the smpd1^−/− mutant zebrafish line. (A) Representative genotyping gel of the smpd1^−/− alleles (5 bp deletion and 136 bp insertion) demonstrating WT, smpd1^+/− and smpd1^−/−. (B) Acid sphingomyelinase (ASM) enzymatic activity in smpd1^−/− compared to WT controls (n=6 larvae at 5 dpf per genotype, P=0.006 by two-tailed unpaired Welch's t-test). (C) Quantification of sphingolipid metabolites, namely, sphingomyelin (Sph), dihydro-sphingomyelin (DHSph), ceramide (Cer), lactosylceramide (LacCer), sphinganine (Spgn) and sphinganine 1 phosphate (Sa1P). All metabolites shown are the C18 neuronal species. Data represented are the mean±s.d. *P<0.05; **P<0.01; ***P<0.001 (two-tailed unpaired t-test).

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.