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Fig. 1
Figure 1. SNRNP70 localizes to axons (A) A representative image of a dissociated motor neuron at 1 day in vitro derived from Tg(mnx1:GFP) animals at 28 hpf. The sample was stained with anti-SNRNP70 to reveal the localization of the protein in neurites and DAPI to mark nuclei. Scale bars, 10 μm. (B) Inset showing a higher magnification of the motor axon in (A). Arrows indicate SNRNP70+ puncta within the GFP+ axon. Scale bars, 5 μm. (C) Time-lapse of neurons at 48 hpf sparsely labeled with hSNRNP70-eGFP. Top: images show GFP-labeled neurons co-expressing UTP-Cy5. Bottom: insets depict axon segments showing association between Cy5-RNA granules (magenta) and hSNRNP70-eGFP (green) signals (arrows indicate the same punctum during the imaging session with time shown in the bottom right corner of individual time points). In the single-channel images, the hSNRNP70-eGFP fluorescence signal has been outlined and overlayed in the UTP-Cy5 signal for a direct comparison. Representative examples from three independent experiments. Scale bars, 5 μm. See also Figure S1 and Video S1.