Fig. 8

Fig. 8 Fig. 8. Treatment with GLX351322 impairs Saos-2 cell proliferation and differentiation. (A) After culture for 12 days, alizarin red staining was performed in control, induced, and GLX351322-treated/induced cells. (B) Saos-2 cells in the control, induction, and induction + GLX351322 groups from days 1–4, 5–8, and 9–12, respectively, were stained with alizarin red on culture day 12. (C) RT-qPCR was used to analyze Runx2, Osx, and Ocn expression in Saos-2 cells treated with GLX351322 from days 1–4, 5–8, and 9–12. (D) Cells in the control, induction, GLX351322 + induction, and GLX351322 + SB431542 + induction groups were stained with alizarin red after 12 days of culture. The results were analyzed using two-way ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)