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Fig. 2 Fig. 2. Construction of nox4−/− zebrafish. (A) The structure of zebrafish nox4 and the guide RNA (gRNA) site. The gRNA targets a sequence in exon 4. (B) Two homozygous mutant lines were obtained and identified by PCR. Lane 1: DNA marker; Lane 2: WT PCR product; Lane 2: PCR product from the −4 bp mutant; Lane 4: PCR product from the +142 bp mutant. (C) Nucleic acid sequences of two nox4 homozygous mutant lines showing a 4-bp deletion or a 142-bp increase in the homozygous mutants. (D) Sequencing map of nox4 mutant lines.
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Reprinted from Free radical biology & medicine, 193(Pt 2), Cao, Z., Liu, G., Zhang, H., Wang, M., Xu, Y., Nox4 promotes osteoblast differentiation through TGF-beta signal pathway, 595-609, Copyright (2022) with permission from Elsevier. Full text @ Free Radic. Biol. Med.