ZFIN Image: Kondo et al., 2023, Figure 3

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Figure 3

Serial transplantation assay for wild-type and jam1a^sd4 KMCs

(A) Experimental procedure for serial transplantation assay. DsRed-labeled KMCs from wild-type or jam1a^sd43 animals were co-transplanted with equivalent numbers of BFP-labeled wild-type KMCs into sub lethally irradiated wild-type recipients (wild-type or jam1a^sd43 group). At 4 weeks post-transplantation (wpt), KMCs from each recipient group were analyzed by FCM and further transplanted into secondary recipients, followed by FCM analysis at 6 wpt.

(B and C) Representative result of FCM analysis in recipients of wild-type or jam1a^sd43 group.

(D) Percentages of dsRed⁺ cells within the total BFP⁺ or dsRed⁺ cells in each group (mean ± s.e.m; n = 35 (wild-type) or 33 (jam1a^sd43) for primary; n = 14 (wild-type) or 16 (jam1a^sd43) for secondary). ∗p < 0.05; ∗∗p < 0.01.

Acknowledgments

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