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Fig. 1 (A) Genetic crosses used to obtain Ex0002[act-3p::rfp] wild-type offspring from act-5(dt2019);Ex0002[act-3p::rfp] heterozygous nematodes. Black arrows indicate the self-fertilization of hermaphrodites; red arrows indicate subsequent wild-type self-crosses. (B) Percentage of adult Ex0002[act-3p::rfp] wild-type nematodes displaying ectopic red fluorescent protein (RFP) expression in their intestine through six generations from act-5(dt2019/+);Ex0002[act-3p::rfp] ancestors. Red numbers indicate the total number of Ex0002[act-3p::rfp] wild-type nematodes displaying ectopic RFP expression in their intestine in each generation; black numbers indicate the total number of Ex0002[act-3p::rfp] wild-type nematodes displaying pharynx-only RFP expression in each generation. (C) Genetic crosses used to obtain Ex1001[act-3p::rfp] offspring from Si[eft-3p::act-5(ptc)](tg/+);Ex1001[act-3p::rfp] nematodes. Black arrows indicate the self-fertilization of hermaphrodites; red arrows indicate subsequent wild-type self-crosses. (D) Percentage of adult Ex1001[act-3p::rfp] wild-type nematodes displaying ectopic RFP expression in their uterus through nine generations from Si[eft-3p::act-5(ptc)](tg/+);Ex1001[act-3p::rfp] ancestors. Red numbers indicate the total number of Ex1001[act-3p::rfp] wild-type nematodes displaying ectopic RFP expression in their uterus in each generation; black numbers indicate the total number of Ex1001[act-3p::rfp] wild-type nematodes displaying only pharynx and spermatheca RFP expression (fig. S1D) in each generation. Illustrations were created with BioRender.com.