ZFIN Image: Saelens et al., 2022, Fig. 6

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Figure Caption

Fig. 6

Altered macrophage motility and morphology during M. tuberculosis infection with full-length EsxM variants

(A) Time-lapse imaging of BLaER1 cells infected with Mtb₆₀₂₀ msp12?cerulean or Mtb₆₀₂₀ hsp60p?esxM/msp12?cerulean. Bacteria false-colored red. Spiky projections, likely retraction fibers, (blue arrowheads) localize to the lagging edge in BLaER1 cells infected with Mtb₆₀₂₀ msp12?cerulean. Filopodial projections (green arrowheads) localize to the leading edge in BLaER1 cells infected with Mtb₆₀₂₀ hsp60p?esxM/msp12?cerulean. Black arrows indicate the direction of cell migration.

(B and C) Infection of BLaER1 cells with Lineage 4 (L4) Mtb H37Rv results in infected cells with membrane ruffles. Infection with the Lineage 1 (L1) Mtb NCG outbreak strain results in an increase in spiky projections and (C) a decrease in circularity, two-tailed paired Student?s t test, mean � SD shown. ****p < 0.0001. All images representative from at least two biologically independent experiments.

See also Figures S4 and S5 and Video S4.

Acknowledgments

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Reprinted from Cell, 185(24), Saelens, J.W., Sweeney, M.I., Viswanathan, G., Xet-Mull, A.M., Jurcic Smith, K.L., Sisk, D.M., Hu, D.D., Cronin, R.M., Hughes, E.J., Brewer, W.J., Coers, J., Champion, M.M., Champion, P.A., Lowe, C.B., Smith, C.M., Lee, S., Stout, J.E., Tobin, D.M., An ancestral mycobacterial effector promotes dissemination of infection, 4507-4525.e18, Copyright (2022) with permission from Elsevier. Full text @ Cell