ZFIN Image: Saraswathy et al., 2022, Fig. 3

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Figure Caption

Fig. 3

Cell proliferation in mstnb mutant zebrafish

(A) Experimental timeline to assess the rates of cell proliferation. mstnb^−/− and wild-type siblings were subjected to SC transections. Animal numbers are indicated for each genotype, and two independent replicates are shown.

(B and C) Immunohistochemistry for EdU, HuC/D, and Sox2 in SC sections of mstnb^+/+ and mstnb^−/− at 7 dpi. EdU and HuC/D colocalization is shown in (B). EdU and Sox2 colocalization is shown in (C). Dotted ovals delineate central canal edges. Regions in the rectangular boxes are shown at high magnification. Arrowheads indicate HuC/D^>+ EdU^>+ neurons in (B) and Sox2⁺ EdU^>+ progenitors in (C).

(D) Regenerated HuC/D^>+ EdU^>+ neurons were quantified in D SC sections at 7, 14, or 21 dpi and uninjured controls. Cross-SC sections 450 μm rostral to the lesion site were quantified. The proportions of HuC/D^>+ EdU^>+ neurons (%) were normalized to the numbers of HuC/D^>+ neurons for each section.

(E) Sox2⁺ EdU^>+ progenitors were quantified in D SC sections at 7, 14, or 21 dpi and uninjured controls. Cross-SC sections 450 μm rostral to the lesion site were quantified. The proportions of Sox2⁺ EdU^>+ progenitors were normalized to the numbers of Sox2⁺ progenitors for each section.

Error bars depict SEM, and statistical significance was determined by two-way ANOVA. ANOVA p values and multiple-comparisons p values are indicated. **p < 0.01; ns, p > 0.05. Scale bars, 50 μm.

Acknowledgments

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