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FIGURE 6
Myelin gene expression is normal in Sarm1 null peripheral nerves. (A) Relative mRNA expression for chemokine, Schwann cell injury and myelin genes in the P60 uninjured tibial nerve of Wt and Sarm1 KO mice. All fold change values normalized to uninjured Wt tibial nerve (n = 4; *p < 0.05, **p < 0.01). (B) Representative Western blot image of tibial nerve protein extracts from P60 Wt and Sarm1 KO mice. The image shows no difference in levels of EGR2 and JUN, between Wt and Sarm1 KO nerves. (C) There is no significant difference in EGR2 levels in Wt and Sarm1 KO nerves (n = 7; p = 0.9118). EGR2 protein levels are normalized to the levels in Wt nerves, which are set as 1. (D) There is no significant difference in JUN levels in Wt and Sarm1 KO nerves (n = 7; p = 0.5787). The quantifications are normalized to the levels in Wt nerves, which are set as 1. (E) Representative Western blot image showing no difference in levels of Myelin protein zero (MPZ) and Myelin basic protein (MBP), between P60 Wt and Sarm1 KO nerves. (F) There is no significant difference in MPZ levels in Wt and Sarm1 KO nerves (Wt n = 6; KO n = 5; p = 0.3176). The quantifications are normalized to the levels in Wt nerves, which are set as 1. (G) There is no significant difference in MBP levels in Wt and Sarm1 KO nerves (n = 7; p > 0.9999). The quantifications are normalized to the levels in Wt nerves, which are set as 1.