ZFIN Image: Snodgrass et al., 2023, Fig. 3.

Image

Figure Caption

Fig. 3.

Vegf inhibition between 2 and 3 dpf rescues the abnormal trunk and cerebral vessel phenotypes of eng^mu130 mutants. (A) Experimental plan for rescuing the eng mutant phenotype in zebrafish using AV951. dpf, days post fertilisation. (B) Representative maximum-intensity projection of the trunk vasculature of eng^mu130 and WT embryos±AV951 (25 nM) treatment for 24 h. DA, dorsal aorta; PCV, posterior cardinal vein. Scale bar: 150 μm. (C) Representative maximum-intensity projection of the cerebral vasculature of eng^mu130 and WT embryos±AV951 (25 nM) treatment for 24 h. Scale bar: 100 μm. (D) DA diameter in eng^mu130 and WT embryos±AV951 treatment. (E) PCV diameter in eng^mu130 and WT embryos±AV951 treatment. (F) Intersegmental blood vessel (ISV) diameters in eng^mu130 and WT embryos±AV951 treatment. (G) Percentage of open ISVs in eng^mu130 and WT embryos±AV951 treatment. (H) Number of kugeln in eng^mu130 and WT embryos±AV951 treatment. (I) BA diameter in eng^mu130 and WT embryos±25 nM AV951 treatment. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant (two-way ANOVA with Tukey post-hoc test, 10 animals/group).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.