ZFIN Image: Hong et al., 2023, Fig 4

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Figure Caption

Fig 4

Double immunofluorescence staining of MHC-II⁺Env38⁺ APCs in leukocytes of zebrafish.

The leukocytes were sorted from spleen, head kidney and peripheral blood of zebrafish stimulated with SVCV (10⁵ TCID₅₀). (A) Cells were labled with mouse anti-Env38 and rabbit anti-MHC-IIα Abs, followed by Alexa Fluor 594-conjugated goat anti-mouse IgG and FITC-conjugated goat anti-rabbit IgG. (B) Cells were labled with mouse anti-Env38 and rabbit anti-IgM Abs, followed by Alexa Fluor 594-conjugated goat anti-mouse IgG and FITC-conjugated goat anti-rabbit IgG. DAPI stain showed the location of the nuclei. Fluorescence images were captured using a Laser scanning confocal microscope (FV3000) with 60 × oil glass.

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