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Fig. 2
Effects of eaf1/2 deficiency on erythrogenesis. A O-dianisidine staining analysis of erythrocytes at 36, 48, 72 and 96 hpf in both eaf1−/− and eaf2−/− embryos and larvae relative to WT (A1-A12). Statistical analysis of O-dianisidine staining results (A13). B, C WISH analysis of the expression of embryonic hemoglobin, hbbe1/hbbe2/hbbe3 in eaf1−/−, eaf2−/−, and WT embryos at 14 hpf (B1-B9) and 24 hpf (C1-C9), respectively. The statistical analysis of WISH hemoglobin gene staining results for eaf1−/−, eaf2−/−, and WT embryos at 14 hpf (B10) and 24 hpf (C10), respectively. Each experiment was repeated at least three times, with similar results for two or three replicates, and a representative result was shown. Data are mean ± SD. A1-A12, ventral view, anterior to the left. B1-B9, dorsal view, anterior to the top. C1-C9, lateral view, anterior to the left. *P < .05, **P < .01, ***P < .001. NS, not significant. Scale bar = 75 μm (A1-A12) and 200 μm (B1- B9, C1- C9)