FIGURE 4

Loss of Gjd2b/Cx35.1 Caused a Dysmorphic Head Feature. (A) Illustration of the zebrafish measurements taken in Image J. (B) Images of 7dpf wild-type TL and gjd2b/Cx35.1^−/− zebrafish larva. Quantification of the (C) body length, (D) head length, and (E) midbrain diameter. Gjd2b ^−/− /Cx35.1^−/− larvae were larger in both body and head length in comparison to the age-matched wild-type larvae. The (F) Head-to-Body ratio and (G) Head-to-Midbrain ratios demonstrated a reduction in the midbrain diameter in gjd2b ^−/− /Cx35.1^−/− larvae relative to their body size, suggesting a developmental alteration. The statistical significance was determined by the Mann-Whitney test, p ≤ 0.05. (C–G) Shows Estimation Plots. The sample size (N) was 19 for WT and gjd2b ^−/− /Cx35.1^−/− larvae.