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Figure 5
Pcdh7b regulates the transcription of dopey2 in zebrafish embryos
(A) Whole-mount immunofluorescence staining detected Dopey2 protein in Ctl MO, P7 MO and P7 TRUN injected embryos at 24 hpf. Scale bar = 500 μm.
(B) In situ hybridization analysis detected the expression of dopey2 in 48 hpf embryos pre-inject Ctl MO or P7 MO. Scale bar = 200 μm.
(C) Real-time fluorescence quantitative PCR analysis detected the relative expression level of dopey2 gene in 48 hpf embryos pre-injected with Ctl MO or P7 MO (mean ± s.e.m, n = 3, Student’s t test: ∗∗p < 0.01).
(D) The top panel represents schematic diagram of dopey2 gene primers for ChIP analysis. The bottom panel showed ChIP analysis results of 48 hpf embryos pre-inject with Ctl MO or P7 MO (mean ± s.e.m, n = 3, Student’s t test: ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05).
(E) In situ hybridization analysis detected the expression of dopey2 in 58 hpf WT embryos or P7 MUT embryos pre-injected with Ctl MO or P7 MO. Scale bar = 200 μm.
(F) Real-time fluorescence quantitative PCR analysis detected the relative expression level of dopey2 gene in 48 hpf WT embryos or P7 MUT embryos pre-injected with P7 MO (mean ± s.e.m, n = 3, Student’s t test: NS = not significant).
(G) ChIP analysis results of 48 hpf WT embryos or P7 MUT embryos pre-injected with Ctl MO or P7 MO (mean ± s.e.m, n = 3, Student’s t test: NS = not significant). Ctl MO: control MO, P7 MO: pcdh7b MO, RNA POL IIA: RNA polymerase II with an unphosphorylated CTD, RNA POL IIO: RNA polymerase II with a phosphorylated CTD, WT: wild type, P7 MUT: pcdh7bmutant.