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Figure 4
Growth retardation in isl2a knockout zebrafish. (A) The Kaplan-Meier survival curve of isl2a+/+ (n = 159), isl2a+/− (n = 173), and isl2a−/− (n = 253) larvae shows differences. (B) Male/female ratios in isl2a+/+ (male/female = 20/6), isl2a+/− (male/female = 42/10), and isl2a−/− (male/female = 18/3). (C) The isl2a variant caused significant growth defects in 41 dpf zebrafish adults. Scale bar = 1 mm. (D) Comparison of developmental indicators, including body length, heart rate, eye size, and ear size, among isl2a-/-, isl2a+/+ and isl2a+/- siblings at 5 dpf. (E) Comparison of three diameter lines of skull among different groups at 5 dpf. (F) Dynamic tracking of the body length of isl2a-/- individuals and their isl2a+/+ and isl2a+/- siblings at 14 dpf, 41 dpf, 77 dpf, and 7.5 mpf. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.