Figure 2
Methodology for quantifying rate of adult fin regeneration
(A–F) Amputated adult caudal fins in zebrafish achieve complete, scar-free regeneration by 4 weeks post-amputation (wpa). In all cases, the fin was imaged prior to amputation (A), immediately following amputation (B) and then once weekly at 1wpa (C), 2wpa (D), 3wpa (E), and 4wpa (F). The distinctive stripe and pigment patterns on the body and anal fin enabled identification of individual animals. Fins were amputated midway, and the caudal peduncle was used to demarcate the proximal extent. Scale bar = 5 mm. (G) Repeat (triplicate) measurements of caudal fin areas in 18 adult fish (n = 18).
See also Figures S3, S4, Tables S1, and S2.