ZFIN Image: Ritter et al., 2023, Figure 5

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Figure Caption

Figure 5

Collagen II trafficking is regulated by Rab8a. (A) Experimental design for mosaic overexpression of WT mCherry-hRAB8A fusion protein in zebrafish chondrocytes. (B) Representative images of Tg(Col2a1α:caax-eGFP) transgenic chondrocytes in WT and rgp1^-/- cartilage (mCherry, magenta; caax-eGFP, green; Col2, white). (C) Quantification of the percentage of cytosolic area occupied by collagen II signal in chondrocytes. (D) Experimental design for mosaic overexpression of DN GFP-hRAB8A fusion protein in zebrafish chondrocytes. (E) Representative images of chondrocytes in WT and rgp1^-/- cartilage (phalloidin, magenta; eGFP, green; Col2, white). Yellow arrowhead identifies intracellular collagen II. (F) Quantification of the percentage of cytosolic area occupied by collagen II signal in chondrocytes. (G) Experimental design for mosaic overexpression of CA GFP-hRAB8A fusion protein in zebrafish chondrocytes. (H) Representative images of chondrocytes in WT and rgp1^-/- cartilage (phalloidin, magenta; eGFP, green; Col2, white). Yellow arrowhead identifies collagen II in GFP^- cells while empty arrowhead identifies collagen II in GFP⁺ cells. (I) Quantification of the percentage of cytosolic area occupied by collagen II signal in chondrocytes. Significance is presented by ***p<0.001; ****p<0.0001; ns, not signficant.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)